XTT Assay of Antifungal Activity

Cell Proliferation Kit II (XTT) Colorimetric assay (XTT based) for the non-radioactive quantification of cell proliferation and viability

Introduction The determination of cellular proliferation, viability and activation are key areas in a wide variety of cell biological approaches. The need for sensitive, quantitative, reliable and automated methods led to the development of standard assays. Such an example is based on the capability of the cells to incorporate a radioactively labeled substance ([3H]thymidine), or to release a r...

Colorimetric Assay for Antifungal Susceptibility

Antifungal Activity of Soil Chitinolytic Bacilli

Abstract Background and Objective: Chitin, which is a linear polymer of N-acetyl glucosamine residues, has been the most abundant polymer in nature after cellulose. In recent decades, Chitinases have received increased attention because of their wide range of applications, especially in biological control against fungi. Material and Methods: the isolation of bacilli producing chitinolyti...

Antifungal activity of coumarins.

The antifungal activity of 40 coumarins was tested against the fungal strains: Candida albicans (ATCC 14053), Aspergillus fumigatus (ATCC 16913) and Fusarium solani (ATCC 36031), using the broth microdilution method. Osthenol showed the most effective antifungal activity among all the compounds tested, with a MIC value of 125 microg/ml for Fusarium solani and 250 micro/ml for Candida albicans a...

Improvement of XTT assay performance for studies involving Candida albicans biofilms.

2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay has been used to study Candida biofilm formation. However, considering that the XTT reduction assay is dependent on cell activity, its use for evaluating mature biofilms may lead to inaccuracies since biofilm bottom cell layers tend to be relatively quiescent at later stages of b...